(When used with Antibodies) the amount of excitation necessary to give off the largest number of photons. Generally the higher the quantum efficiency, the better.

or 3-D plots or Histograms – Data display for flow cytometric data. Terms “histogram” and “plot” can be used for 3 parameter (multivariate) graphs. Also known as Multivariate Plots.

Dyed or colored glass. Works by absorption of unwanted wavelengths. Can fluoresce. Inexpensive—not often used alone.

Instrument compartment that converts the electrical signal (stream of electrons) into numbers. Also known as Analog-Digital Conversion.

Increase of the signal detected to adjust signal intensity.

Instrument compartment that converts the electrical signal (stream of electrons) into numbers. Also known as ADC.

(When used in terms of flow cytometry) Instruments used to measure properties of particles (whole cells, nuclei, chromosomes, diatoms, plankton, bacteria, viruses) by moving these particles through a detection chamber.

Sample from whole cells or nuclei containing more (or less) DNA than a normal cell. Proper term is “DNA Aneuploid”. Has a DI less than 2.0 or greater than 2.0

% of the total cycling population that is aneuploid

Measurement of how strongly an Antibody attaches to only one antigen or epitope.